WHO 2016 Classification of PTLD
The table below depicts the most recent WHO 2016 classification on PTLD.
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Figure 1: PET and CT Scans in Plasmacytoma-Like PTLD, EBV+
The image on the top left shows hypermetabolic foci within the abdomen corresponding to multiple bowel loops. In addition, there were several hypermetabolic foci within the abdominal wall, right pelvis, and left lower pelvis.
The image on the top right shows hypermetabolic anterior mediastinal conglomerate lymph node mass with central necrosis.
The CT at the bottom left additionally shows the mediastinal involvement.
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Figure 2: H&E Low Power in Plasmacytoma-Like PTLD, EBV+
The image on the left shows low power of the entire bowel wall with normal small bowel mucosa on the top with extensive infiltration of the muscularis propria and submucosa by a sheetlike infiltrate of neoplastic cells.
The image on the right shows the neoplastic cells at higher power present in sheets percolating in between the fibers of the muscularis propria.
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Figure 3: H&E High Power in Plasmacytoma-Like PTLD, EBV+
These higher power images depict the infiltrate percolating and destroying the muscularis propria.
The high magnification on the right side shows classic plasmacytic cytomorphology. Note that the neoplastic cells do not exhibit morphology compatible with centroblasts (as in DLBCL) or plasmablasts (plasmablastic lymphoma), both of which may also be EBV positive.
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Figure 4: Touch Preperation in Plasmacytoma-Like PTLD, EBV+
Touch preparations demonstrate scattered plasma cells within the lesion.
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FIgure 5: CD20 & CD3 in Plasmacytoma-Like PTLD, EBV+
CD20 (on the left) is negative in the neoplastic cells. Rare CD20 positive small B-cells are seen within the infiltrate.
CD3 (on the right) shows scattered T-cells.
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Figure 6: CD79a & MUM1 in Plasmacytoma-Like PTLD, EBV+
Both CD79a (B-cell/plasmacytic marker on the left) as well as MUM1 (plasmacytic marker on the right) are strongly positive confirming plasmacytic differentiation.
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Figure 7: CD30 & CD56 in Plasmacytoma-Like PTLD, EBV+
A significant proportion of the neoplastic cells are positive for CD30 (pictured on the left) but negative for CD56 (on the right).
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Figure 8: EBER in Plasmacytoma-Like PTLD, EBV+
The neoplastic cells are strongly positive for EBER as seen in this low magnification and high magnification.
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Figure 9: Kappa & Lambda in Plasmacytoma-Like PTLD, EBV+
The infiltrate demonstrates kappa light chain restriction as noted on the left side in both images. Kappa light chain in situ hybridization is represented in the image on the left side. Also note that the normal small bowel mucosa pictured on the right side in both images demonstrates polytypic plasma cells as seen in both the stains on in situ hybridization.
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